3-Hour Plated Hepatocyte Method

SUBTYPE:Hepatobiliary Disposition
APPLICATION & SPECIALITY USES:ADME-Toxicology Products and Services
Keywords:B-CLEARB-CLEAR TechnologyBiliary ClearanceBiliary ExcretionHepatic Clearance

An oil spin study can be use for the following types of evaluations:

  • Rate of hepatic uptake
  • Hepatic accumulation
  • Uptake DDIs and other interaction effects

We typically implement 3-hour plated hepatocyte studies in a 96-well format. The test article is typically incubated with the following control compounds: Rosuvastatin, TCA and Metformin.

Study protocols may be adapted based on the study objectives and characteristics of the test article, but typically include the following conditions:

  • 1 µM
  • 6 timepoints (0.25 to 10 minutes)
  • 37° and 4° C incubations
  • With or without inhibitors (Rif SV, CsA, quinidine)

Deliverables from an oil spin suspension study may include the following data summaries:

  • Uptake (pmol/Mcells) or (pmol/mg)
  • Uptake rate if more than 3 timepoints are included
  • Intrinsic clearance (Cluptake)
  • Kp