3D Human Cell-based Assays

Type:Services
SUBTYPE:Cell Biology/Disease Modeling
APPLICATION & SPECIALITY USES:Biomarker DiscoveryCell Therapy/ Regenerative MedicinePersonalized Medicine
Keywords:3D cell based assay3D cell cultureCell based assayOrgan Models

OPTIONS:

Three dimensional cell cultures are increasingly the method of choice for modeling cell behavior and  investigating the effects of pre-clinical development compounds. In particular, multicellular 3D cell culture formats, often utilizing extracellular matrix (ECM) components for cell attachment / microenvironment influence, allow cells to form multi-layered structures or aggregates. There is increasing evidence that primary cells maintained in a 3D environment rather than conventional 2D culture more faithfully resemble the behavior and responsiveness of cells seen in intact tissues. PHASEZERO® provides established 3D cell culture platforms and 3D cell-based assay development, designing customized assays and relevant biological endpoints to meet specific project needs.

BioIVT’s ORGANDOT® Model

The ORGANDOT™ platform, a three-dimensional (3D) tissue culture system model, was originally developed to evaluate the effects of compounds developed for the treatment of diabetes. Our PHASEZERO™ Research team has now expanded the use of the model for liver fibrosis and cancer biology.

ORGANDOT CREATION

ORGANDOT Creation

  • Dissociation of primary tissue / stem-cell material
  • Transfer to a semi-permeable membrane and culture at air liquid interface
  • Re-aggregation of the cells over time in culture
  • 3D microtissues recapitulate original tissue architecture and functionality

 

 

 

Relevant 3D models include:

3D Pancreatic isletORGANDOT Model

3D Liver Fibrosis ORGANDOT Model

3D Cancer ORGANDOT Model

3D Respiratory Model

AVAILABLE CULTURE FORMATS
• 2D Multiwell plates (6-96-well)
• 2D Transwells
• 3D Air-liquid interface
• 3D ORGANDOT™ matrix free aggregates
• Experience with additional 3D platforms, including Spheroid (with and w/o soft matrix), collagen constructs, hanging drop, magnetically aggregated models and more

ASSAY TYPES / ENDPOINTS
• Cytokine / mediator release
• Cell viability / proliferation
• Apoptosis
• Changes in gene expression
• Enzyme activity / release
• Barrier permeability
• Cell migration
• IHC / ICC