3D Human Cell-based Assays


SUBTYPE:Cell Biology/Disease Modeling
APPLICATION & SPECIALITY USES:Biomarker DiscoveryCell Therapy/ Regenerative MedicinePersonalized Medicine


SKU: RPZ3CELL Categories: ,

Three dimensional cell cultures are increasingly the method of choice for modeling cell behavior investigation of the effects of pre-clinical development compounds. In particular, multicellular 3D cell culture formats, often utilizing extracellular matrix (ECM) components for cell attachment / microenvironment influence, allow cells to form multi-layered structures or aggregates. There is increasing evidence that primary cells maintained in a 3D environment rather than conventional 2D culture more faithfully resemble the behavior and responsiveness of cells seen in intact tissues. PHASEZERO® provides established 3D cell culture platforms and 3D cell-based assay development, designing customized assays and relevant biological endpoints to meet specific project needs.

Relevant models include:

Human Pancreatic Islets for the study of Type 2 diabetes

Using our proprietary OrganDOT™ platform, our PHASEZERO scientists developed the isletOrganDOT model

  • Prepared from readily available, high-quality fresh human pancreatic islets
  • Recapitulates islet morphology, gene expression and function
  • Consistent GSIS responses achieved in OrganDOT cultures from over 50 donors together with reliable standard secretagogue-mediated potentiation of GSIS
  • Valuable for studying a variety of pharmacologically-relevant mechanisms, including characterization of novel GPR40 agnoists
  • Long-term viability or OrganDOT cultures enables sequential or chronic compound testing

Human Bronchial Epithelial Cells for the study of respiratory function

Our PHASEZERO scientists have extensive experience in the preparation of organotypic cultures of human bronchial epithelium, using air-liquid interface (ALI) conditions to generate highly differentiated, mucocillary cultures that mimic many of the features of the airway epithelium.

  • Human bronchial epithelial cells (huBEC) prepared from high quality fresh lung
  • Cryopreserved HuBEC inventory for rapid project turnaround
  • Established assay endpoints include: Release of inflammatory mediators, mucous (goblet) cell hyperplasia, mucus secretion, squamous cell development, changes in gene expression, changes in barrier function
  • HuBECs available from donors without respiratory disease and from donors with asthma, COPD or cystic fibrosis (genotyped)
  • Potential to reveal differential effects of novel therapeutics on cells from non-diseased and diseased donors
    • Primary Human Liver cells for liver fibrosis studies
    • Primary Human Cancer Associated Fibroblasts and Epithelial Cells as well as established tumor cell lines for oncology models

Features & Benefits

The advancements in functionality, robustness, throughput, and study design enabled by the isletOrganDOT model provide relevant human data on the effects of test compounds on islet function in a rapid and reproducible manner.

Advantages of the OrganDOT™ System

  • 3D microtissues recapitulate original tissue architecture and functionality
  • Robust function, longevity
  • Compatible with multiple endpoint assays such as: mediator release, cell viability/proliferation/apoptosis, ICC & qRT-PCR
  • Simultaneous testing of multiple treatments and schedules delivers comprehensive compound profiles within a shorter time period
  • Established isletOrganDOT™
  • Potential to be applied to different cell type systems
    • Liver (hepatocyte, stellate and Kupffer cell co-culture)
    • Lung (tumor cells + CAFs co-culture)