Verified Efflux Transporter Vesicles

Type:Uncategorized
SUBTYPE:Transporter Products
APPLICATION & SPECIALITY USES:ADME-Toxicology Products and Services
  • Proven, high-quality ABC transporter vesicles manufactured by GenoMembrane
  • Quality assured — functionality of every batch verified by BioIVT
  • Unmatched technical support from BioIVT

BioIVT provides transporter vesicle products  manufactured by GenoMembrane Co., Ltd. (Japan) to clients in the US and Canada. These “inside-out” vesicles are ideal for studying efflux transporters, and can be used to complement BioIVT’s cell-based TRANSFLEX™ Plates.

BioIVT provides assurance of product quality by performing rigorous functional testing on each batch of products.

 

Available Products
TransporterSpeciesBioIVT Cat. #
BCRP (ABCG2)HumanOPT‐VGM0008
RatOPT‐VGM0007
BSEP (ABCB11)HumanOPT‐VGM0005
RatOPT‐VGM0006
DogOPT‐VGM0019
MDR1/P-gp (ABCB1)HumanOPT‐VGM0015
Mouse—Mdr1aOPT‐VGM0004
Mouse—Mdr1bOPT‐VGM0016
MRP1 (ABCC1)HumanOPT‐VGM0010
RatOPT‐VGM0011
DogOPT‐VGM0017
MRP2 (ABCC2)HumanOPT‐VGM0001
RatOPT‐VGM0002
DogOPT‐VGM0014
MouseOPT‐VGM0022
MonkeyOPT‐VGM0018
MRP3 (ABCC3)HumanOPT‐VGM0021
MRP4 (ABCC4)HumanOPT‐VGM0012
RatOPT‐VGM0020
MRP8 (ABCC8)HumanOPT‐VGM0013
P-gp/MDR1HumanOPT‐VGM0015
Mouse—Mdr1aOPT‐VGM0004
Mouse—Mdr1bOPT‐VGM0016
ControlOPT‐VGM0003

 

Why ABC Transporter Vesicles?
  • Easily assess your compound’s interactions with efflux transporters
  • Convenient, cell-free system
  • High signal-to-noise ratios
  • Satisfy EMA guideline recommendations for secondary (non-cell-based) systems

ATP-Binding Cassette (ABC) Transporter Vesicles allow easy evaluation of compound interactions with a given efflux transporter. These inverted/inside-out vesicles are prepared from Sf9 insect cells engineered to overexpress individual efflux transporters. In cell-based systems, efflux proteins typically transport compounds out of the cells and into the environment. In an inverted vesicle format, the proteins are re-oriented along with the inverted membranes. As a result, they bind to the substrate in the solution surrounding the vesicles, and transport it into the vesicles where it accumulates. Quantitation of the accumulated compound allows determination of whether the compound acts as a substrate and/or inhibitor of the transporter in question. The high levels of transporter activity and low background activity associated with these vesicles typically result in high signal-to-noise ratios, providing clear, unambiguous results. Please note, however, that highly permeant compounds may diffuse into or out of the vesicles, independent of transporter activity, minimizing differences between transporter expressing and control vesicles, potentially resulting in false negative results.

In addition to being a convenient, cell-free system vesicular systems provide a secondary system that can be used to satisfy the EMA’s recommendation for the assessment of P-gp inhibition, and possibly that of other transporters. According to EMA guidelines1, because of the high inter-laboratory variability in the estimation of P-gp inhibition, “the use of two separate systems is recommended for this transporter…. [and] could also be considered for other transporters if indicated.”

Reference:

1 Guideline on the Investigation of Drug Interactions