ASBT (SLC10A2) Transporter Assay

The apical sodium-dependent bile-acid transporter (ASBT) is a member of the sodium/bile-salt co-transport family coded by gene SLC10A2. The transporter is electrogenic, coupled with sodium in a 2:1 sodium:bile acid stoichiometry. Human ASBT presumably functions as a monomer, although some evidence suggests the existence of a dimer as well.  ASBT is currently thought to be the primary mechanism for bile acid reabsorption in the intestine. The transporter is expressed at high levels in the terminal ileum, renal proximal tubules, and biliary epithelium.

BioIVT offers ASBT substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies, from single concentration screens to full kinetic studies for IC50 determinations for ASBT inhibitors, as well as Vmax and Km studies for ASBT substrates.

 

ASBT Transporter Assay Details
Relevance – Bile acid uptake Single or Multi-Transporter Model – Single
Transporter Type – Uptake Cell Model – MDCK-II
Assay Type – Intracellular accumulation Exactly Matched Control Cells– yes
Species – Human Probe Substrate – Taurocholate
Subcellular Localization in Assay Model – Apical Inhibition Positive Control – NaCDC