MATE1 (SLC47A1) Transporter Assay
he MATE1 transporter is the multidrug and toxin extrusion transporter that is encoded by the SLC47A1 gene. MATE1 is expressed in the apical membrane in the kidney and liver and can mediate elimination of substrates.
BioIVT offers MATE1 substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies, from single concentration screens to full kinetic studies for IC50 determinations for MATE1 inhibitors, as well as Vmax and Km for MATE1 substrates.
MATE1 Transporter Assay Details
|Relevance – Biliary secretion and renal tubular secretion
||Single or Multi-Transporter Model – Single|
|Transporter Type – Efflux||Cell Model – MDCK-II|
|Assay Type – Intracellular accumulation||Exactly Matched Control Cells– Yes|
|Species – Human, rat
||Probe Substrate – Metformin
|Subcellular Localization in Assay Model – Apical||Inhibition Positive Control – Cimetidine