MATE1 (SLC47A1) Transporter Assay

he MATE1 transporter is the multidrug and toxin extrusion transporter that is encoded by the SLC47A1 gene. MATE1 is expressed in the apical membrane in the kidney and liver and can mediate elimination of substrates.

BioIVT offers MATE1 substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies, from single concentration screens to full kinetic studies for IC50 determinations for MATE1 inhibitors, as well as Vmax and Km for MATE1 substrates.

 

MATE1 Transporter Assay Details
Relevance – Biliary secretion and renal tubular secretion
Single or Multi-Transporter Model – Single
Transporter Type – Efflux Cell Model – MDCK-II
Assay Type – Intracellular accumulation Exactly Matched Control Cells– Yes
Species – Human, rat
Probe Substrate – Metformin
Subcellular Localization in Assay Model – Apical Inhibition Positive Control – Cimetidine