NET (SLC6A2) Transporter Assay
Norepinephrine (NE) is synthesized from dopamine by dopamine beta-hydroxylase and stored in vesicles. Under normal conditions, neuronal activation promotes the vesicular release of norepinephrine (NE) into the synapse. The norepinephrine transporter (NET; SLC6A2) is responsible for re-uptake of NE into the presynaptic neuron in order to terminate its action. NET transporter is found in central and peripheral neurons, adrenal gland and placenta.
BioIVT offers NET substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies including single concentration screens up to full kinetic studies to determine the IC50 for NET inhibitors as well as Km and Vmax values for NET substrates.
NET Transporter Assay Details
|Relevance – Neurotransmission; validated therapeutic target
||Single or Multi-Transporter Model – Single|
|Transporter Type – Uptake
||Cell Model – MDCK-II
|Assay Type – Intracellular accumulation
||Exactly Matched Control Cells– Yes|
|Species – Human
||Probe Substrate – Dopamine
|Subcellular Localization in Assay Model – Basolateral
||Inhibition Positive Control – Desipramine