OAT3 (SLC22A8) Transporter Assay

The OAT3 transporter is an organic anion uptake transporter that is encoded by the SLC22A8 gene. OAT3 is expressed in the kidney on the basolateral membrane of proximal tubule epithelial cells. Because it transports compounds into the kidney from the bloodstream, OAT3 is involved in the renal elimination of these compounds and their metabolites.

BioIVT offers OAT3 substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies, from single concentration screens to full kinetic studies for IC50 determinations for OAT3 inhibitors, as well as Vmax and Km studies for OAT3 substrates.

 

OAT3 Transporter Assay Details
Relevance – FDA & EMA guidances; renal uptake
Single or Multi-Transporter Model – Single
Transporter Type – Uptake Cell Model – MDCK-II
Assay Type – Intracellular accumulation Exactly Matched Control Cells– Yes
Species – Human, mouse
Probe Substrate – Estrone-3-sulfate
Subcellular Localization in Assay Model – Basolateral
Inhibition Positive Control – Probenecid