OAT4 (SLC22A11) Transporter Assay

The OAT4 transporter is an organic anion uptake transporter that is encoded by the SLC22A11 gene. OAT4 is expressed primarily in the apical membrane in the kidney and can mediate reabsorption of substrates from the urine back into the kidney and potentially the blood stream.

BioIVT offers substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies including single concentration screens up to full kinetic studies to determine the IC50 for OAT4 inhibitors as well as Km and Vmax values for OAT4 substrates.

 

OAT4 Transporter Assay Details
Relevance – Renal tubular reabsorption
Single or Multi-Transporter Model – Single
Transporter Type – Uptake
Cell Model – MDCK-II
Assay Type – Intracellular accumulation
Exactly Matched Control Cells– Yes
Species – Human
Probe Substrate – Estrone-3-sulfate
Subcellular Localization in Assay Model – Apical
Inhibition Positive Control – Indomethacin