OCT1 (SLC22A1) Transporter Assay
OCT1 is an organic cation uptake transporter that is encoded by the SLC22A1 gene. OCT1 is expressed in liver hepatocytes on the basolateral side of the sinusoidal membrane.
BioIVT offers OCT1 substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies, from single concentration screens to full kinetic studies for IC50 determinations for OCT1 inhibitors, as well as Vmax and Km studies for OCT1 substrates.
OCT1 Transporter Assay Details
|Relevance – EMA guidance; hepatic uptake
||Single or Multi-Transporter Model – Single|
|Transporter Type – Uptake||Cell Model – MDCK-II|
|Assay Type – Intracellular accumulation||Exactly Matched Control Cells– Yes|
|Species – Human, rat
||Probe Substrate – Metformin or MPP+
|Subcellular Localization in Assay Model – Basolateral
||Inhibition Positive Control – Quinidine