OCT1 (SLC22A1) Transporter Assay

OCT1 is an organic cation uptake transporter that is encoded by the SLC22A1 gene. OCT1 is expressed in liver hepatocytes on the basolateral side of the sinusoidal membrane.

BioIVT offers OCT1 substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies, from single concentration screens to full kinetic studies for IC50 determinations for OCT1 inhibitors, as well as Vmax and Km studies for OCT1 substrates.


OCT1 Transporter Assay Details
Relevance – EMA guidance; hepatic uptake
Single or Multi-Transporter Model – Single
Transporter Type – Uptake Cell Model – MDCK-II
Assay Type – Intracellular accumulation Exactly Matched Control Cells– Yes
Species – Human, rat
Probe Substrate – Metformin or MPP+
Subcellular Localization in Assay Model – Basolateral
Inhibition Positive Control – Quinidine