OCT2 (SLC22A2) Transporter Assay

OCT2 is an organic cation uptake transporter that is encoded by the SLC22A2 gene. OCT2 is expressed in the kidney on the basaolateral membrane of proximal tubule epithelial cells. Because it transports its substrates from the bloodstream into the kidney, OCT2 is involved in the renal elimination of these compounds.

BioIVT offers OCT2 substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies, from single concentration screens to full kinetic studies for IC50 determinations for OCT2 inhibitors, as well as Vmax and Km studies for OCT2 substrates.


OCT2 Transporter Assay Details
Relevance – FDA & EMA guidance; renal uptake
Single or Multi-Transporter Model – Single
Transporter Type – Uptake Cell Model – MDCK-II
Assay Type – Intracellular accumulation Exactly Matched Control Cells– Yes
Species – Human, mouse, rat, monkey (cynomolgus)
Probe Substrate – Metformin
Subcellular Localization in Assay Model – Basolateral
Inhibition Positive Control – Quinidine