OCTN2 (SLC22A5) Transporter Assay
The OCTN2 transporter is a cation/zwitterion sodium dependent high affinity carnitine transporter. It is localized widely in many organs including the kidneys, pancreas, prostate, skeletal muscle and heart. Genetic defects affecting OCTN2 are responsible for conditions including hypoglycemia and carnitine deficiency, which can lead to rhabdomyolysis, cardiomyopathy and heart failure.
BioIVT offers OCTN2 substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies including single concentration screens up to full kinetic studies to determine the IC50 determinations for OCTN2 inhibitors, as well as Vmax and Km studies for OCTN2 substrates.
OCTN2 Transporter Assay Details
|Relevance – Renal tubular reabsorption, critical genetic polymorphism
||Single or Multi-Transporter Model – Single|
|Transporter Type – Uptake||Cell Model – MDCK-II|
|Assay Type – Intracellular accumulation||Exactly Matched Control Cells– Yes|
|Species – Human
||Probe Substrate – Carnitine
|Subcellular Localization in Assay Model – Apical
||Inhibition Positive Control – Verapamil