PCFT (SLC46A1) Transporter Assay

The Proton-Coupled Folate Transporter, PCFT, is a proton-folate symporter that couples the uptake of folates to the flow of protons down an electrochemical gradient. PCFT is a member of the major facilitator superfamily (MFS) of transporters; it functions optimally at acidic pH, and is primarily involved in the intestinal absorption of dietary folates. PCFT is expressed at high levels in the apical membranes of the duodenum and proximal jejunum, and at much lower levels in the rest of the intestine and colon. The protein is also expressed in other normal tissues, such as liver and kidney. PCFT can function in the absence of a transmembrane pH gradient; however, under these conditions, folate transport is driven by membrane potentials.

BioIVT offers PCFT substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies including single concentration screens up to full kinetic studies to determine the IC50 determinations for PCFT inhibitors, as well as Vmax and Km studies for PCFT substrates.

 

PCFT Transporter Assay Details
Relevance – Vitamin transport
Single or Multi-Transporter Model – Single
Transporter Type – Uptake
Cell Model – MDCK-II
Assay Type – Intracellular accumulation
Exactly Matched Control Cells– Yes
Species – Human
Probe Substrate – Methotrexate
Subcellular Localization in Assay Model – Apical
Inhibition Positive Control – Bromosulfophthalein