PEPT2 (SLC15A2) Transporter Assay

PEPT2 is a high affinity, low capacity transporter that is encoded by the SLC15A2 gene. It is expressed in the apical membrane of the kidney and the choroid plexus. It facilitates renal reabsorption and distribution into the choroid plexus for substrates.

Optivia offers PEPT2 substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies including single concentration screens up to full kinetic studies to determine the IC50 determinations for PEPT2 inhibitors, as well as Vmax and Km studies for PEPT2 substrates.

 

PEPT2 Transporter Assay Details
Relevance – Renal tubular reabsorption
Single or Multi-Transporter Model – Single
Transporter Type – Uptake
Cell Model – MDCK-II
Assay Type – Intracellular accumulation
Exactly Matched Control Cells– Yes
Species – Human
Probe Substrate – Gly-Sar
Subcellular Localization in Assay Model – Apical
Inhibition Positive Control – Losartan