SMVT (SLC5A6) Transporter Assay
SMVT is the sodium dependent multivitamin transporter. It is widely expressed in many tissues including the intestines, kidney, heart, lung and the blood-brain barrier.
Creating biotinylated conjugates has been used as a strategy for drugs such as HIV protease inhibitors to exploit SMVT to achieve higher permeability into select organs and penetration across the blood-brain barrier (BBB). SMVT over expressions has been shown in some tumor cells, providing a possible route for increased uptake and targeted distribution of oncology drugs.
BioIVT offers SMVT substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies including single concentration screens up to full kinetic studies to determine the IC50 for SMVT inhibitors as well as Vmax and Km values for SMVT substrates.
SMVT Transporter Assay Details
|Relevance – Vitamin transport
||Single or Multi-Transporter Model – Single|
|Transporter Type – Uptake
||Cell Model – MDCK-II
|Assay Type – Intracellular accumulation
||Exactly Matched Control Cells– Yes|
|Species – Human
||Probe Substrate – Biotin (vitamin B7)
|Subcellular Localization in Assay Model – Apical
||Inhibition Positive Control – Pantothenate (vitamin B5)