SMVT (SLC5A6) Transporter Assay

SMVT is the sodium dependent multivitamin transporter. It is widely expressed in many tissues including the intestines, kidney, heart, lung and the blood-brain barrier.

Creating biotinylated conjugates has been used as a strategy for drugs such as HIV protease inhibitors to exploit SMVT to achieve higher permeability into select organs and penetration across the blood-brain barrier (BBB). SMVT over expressions has been shown in some tumor cells, providing a possible route for increased uptake and targeted distribution of oncology drugs.

BioIVT offers SMVT substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies including single concentration screens up to full kinetic studies to determine the IC50 for SMVT inhibitors as well as Vmax and Km values for SMVT substrates.

 

SMVT Transporter Assay Details
Relevance – Vitamin transport
Single or Multi-Transporter Model – Single
Transporter Type – Uptake
Cell Model – MDCK-II
Assay Type – Intracellular accumulation
Exactly Matched Control Cells– Yes
Species – Human
Probe Substrate – Biotin (vitamin B7)
Subcellular Localization in Assay Model – Apical
Inhibition Positive Control – Pantothenate (vitamin B5)