When a sample of blood is spun in a centrifuge, the heavier formed elements are packed down by centrifugal force and the less dense plasma remains at the top. A thin, whitish layer called the buffy coat is found at the erythrocyte-plasma junction. This layer contains leukocytes and platelets. When this process is performed with a whole unit of blood, the resulting product is approximately 100ml of concentrated white blood cells mixed with reduced levels of plasma and red blood cells. This makes it an ideal starting material for isolation of blood-derived mononuclear cells.
BioIVT collects human whole blood at our own FDA registered donor centers. Following optimized Standard Operating Procedures, we can ship buffy coat units for next day delivery.