Kits contain everything needed to perform an assay including:
Human HEPATOMUNE® cultures in a 24-well or 96-well format
Maintenance medium for both pre and post Kupffer cell augmentation
Application-specific media and additives
Instructions for culture maintenance and application-specific protocols
Why Use HEPATOMUNE Kits
The HEPATOMUNE culture is a highly functional in vitro liver platform that models inflammation-mediated hepatotoxicity. Its proprietary patterning of hepatocyte “islands” in a “sea” of stromal and Kupffer cells mimic the physiological microenvironment of the liver, enabling innate response to cytokine-mediated drug-induced liver injury. Its proven longevity of at least 10 days provides more than adequate experimentation time to analyze the inflammation response.
The Human HEPATOMUNE kits is designed for numerous applications including:
Evaluation of inflammation-mediated hepatotoxicty across multiple endpoints, such as ALT, ATP, Urea and GSH
Retention of long-term cultures (10 days) for testing hepatotoxicity of compounds and multiple dosing scenarios
Determination of mechanistic information
Kits are backed by scientific support and our quality assurance guarantee. Prior to preparing a kit for shipment we set up a teleconference call with our clients to understand their research objectives and applications. Throughout the process our scientific team is available to provide technical support on experimental design, set up, and data analysis. We are committed to working with our clients to ensuring they can successfully implement studies using HEPATOMUNE kits.
Order Process and Kit Preparation
Typically, customers receive HEPATOMUNE kits 14 days after placing an order. When we receive a kit order we schedule a teleconference call with the researcher(s) who will use the kit to review the research objectives, applications, as well as intended study timing. We will provide research protocols and reference documents that may be helpful.
The HEPATOMUNE co-cultures are developed in our lab using our proprietary micropatterned technology.
Dosing can begin 2 days after receiving a kit. Cultures remain viable for up to 10 days.