Custom Cell-based Assays


SUBTYPE:Cell Biology/Disease Modeling
APPLICATION & SPECIALITY USES:Biomarker DiscoveryCell Therapy/ Regenerative MedicinePersonalized Medicine
Keywords:2D cell culture3D cell cultureCell Culture AssayCell-based Assay DevelopmentCustom Cell-based Assay


Our BioIVT PHASEZERO® scientists has established expertise in the isolation and culture of human primary cells and in the development of human primary cell-based assays (both 2D and 3D) to support late lead optimization/candidate selection stage compound evaluation. Non-human tissues and cultured cells and human recombinant cell lines are used effectively for screening and the initial identification of compounds with desired pharmacological effects. Moreover, using human tissues and primary cells ultimately provides translational data to support the selection of those compounds with the best opportunity for success in the clinic.

Human Tissue Types

Our core cell-based assay disease areas are inflammation, respiratory and metabolic, with development of 2D and 3D cell culture models ongoing in oncology and fibrosis.

Additional examples of primary cells that have been isolated for custom projects include:

  • Blood – PBMC, monocytes, lymphocytes and neutrophils
  • Skin – Keratinocytes, fibroblasts
  • Lung – Alveolar macrophages, alveolar type II cells, smooth muscle cells, tumor epithelial cells, cancer-associated fibroblasts
  • Kidney – Proximal tubule cells
  • Breast – Tumor epithelial cells, cancer-associated fibroblasts
  • Colon – Tumor epithelial cells, cancer-associated fibroblasts

Features & Benefits

Ability to isolate primary cells from a variety of tissue types and representative diseases 2D and 3D models available Culture Formats include:

  • 2D-Multiwell (6-96 well format)
  • 2D-Transwells
  • 3D-Spheroid (with or without soft matrix)
  • 3D-Air-liquid interface (ALI)
  • 3D-OrganDOT™ matrix free aggregates
  • 3D-Raft™ physiological density matrix