Join us at the 12th International ISSX Meeting, July 28-31, 2019 at the Oregon Convention Center. Discover our leading portfolio of hepatic products and research services and learn how we help can help you accelerate your R&D programs, gain regulatory approval, and achieve your commercial objectives. Stop by our booth # 301 to enter to win a copy of “Experimental Cholestasis Research”, co-authored by Kenneth R. Brouwer, PhD, RPh, Vice President, ADME-Tox, BioIVT.
See our schedule of scientific contributions below.
Tuesday, July 30th, 4:30 – 5:30 pm
Potential Benefits of a Physiologically-Relevant Whole Cell Model to Assess CYP Inhibition with Complex Mixtures
Speaker: Amy L. Roe, PhD, DABT, Principal Toxicologist, Product Safety & Regulatory Affairs, The Procter and Gamble Company
Dr. Amy Roe will present results from a published study (Roe et. al., 2019 AIVT) to evaluate potential inhibition of CYP3A4/5 and CYP2C9 enzymatic activity by Boswellia serrata extract (BSE) using pooled human liver microsomes (PHLM) and a more physiologically-relevant sandwich-cultured human hepatocytes (SCHH) model. In the scientific literature, BSE has been shown to reduce the activity (55% to 65%) across major CYP450 enzymes, including CYP3A4/5 and CYP2C9, using baculovirus-infected insect cells. These reported results, contrasted with a seemingly history of safe use of BSE, led us to question the relevance of the PHLM model. We will show that potent CYP3A4/5 and CYP2C9 inhibition observed for BSE in microsomal systems was not observed in SCHH. SCHH are particularly useful for studying complex mixtures such as botanicals and are an effective model to evaluate BDI and DDI.
The Use Of Primary Cryopreserved Human Hepatocytes As A Model For Non-Alcoholic Fatty Liver Disease (NAFLD) Related Changes In Phase I And Phase II Enzyme Activities
Observed vs. Predicted effects Of Protein Binding: Improving In Vivo Predictions of Hepatic Clearance, Intracellular Concentration and IC50 Values
In Vitro Development Of Folate Transport And Endocytosis By PCFT, RFC, and FRα, In A Transwell System
Please join us at the 15th annual assembly of the HRA.
Tuesday, July 29th, 5:30 – 9:00 pm
Portland Ballroom 252-253
Click here to register to attend.