Speak with our experts at 10th Anniversary of the annual Advances in Cell & Tissue Culture (ACTC) at the School of Biosciences at Cardiff University. Our team of scientists and specialists will be present to discuss how we can help accelerate your cell and tissue Culture needs. With the expertise to ethically procure fresh tissues across different normal and disease states, isolate primary and immune cells based on your need, and to perform 2D and 3D custom cell-based assays, as well as capabilities in protein expression such as IHC and multiplex IHC, gene expression such as qRT-PCR, and ADME-TOX, our experts will be there to discuss your needs in depth. See our full schedule of our scientific contributions below at the Advances in Cell and Tissue Culture at the School of Biosciences at Cardiff University.
May 23rd, 8:30 am to 8:50 am
WORKSHOP: Development of a Novel Human 3D In Vitro Model for Evaluating New Anti-Fibrotic Drugs
Caroline Freathy, Ph.D, Principal Scientist, BioIVT
The discovery and development of anti-fibrotic therapies remain heavily reliant on animal testing. There is an urgent need to develop robust and relevant in vitro models to support the identification and preclinical evaluation of potential new anti-fibrotic drugs. To this end, we have developed and characterized a novel human 3D liver co-culture model using our proprietary ORGANDOT™ platform. These liver ORGANDOT cultures (human hepatocytes, Kupffer cells, and stellates) not only maintain viability and functionality for up to four weeks in culture but can also be treated with TGFβ1-t to induce a fibrotic phenotype. The TGFβ1-t-treated ORGANDOT cultures show a decrease in hepatocyte function and a concomitant increase in fibrogenic gene expression (COL1A1, ACTC2, CTGF, OPN, TIMP-2), hyaluronic acid secretion, and collagen deposition (collagen I immunostaining). Furthermore, co-administration of an ALK5 inhibitor was able to completely prevent these fibrotic changes and rescue the functionality of the cultures. These data demonstrate the potential utility of this in vitro model for testing the efficacy of new anti-fibrotic drug candidates or for research into the mechanism of stellate cell activation and fibrosis induction.
May 21st through May 23rd
Poster Title: Development of a Novel Human 3D In Vitro Model for Evaluating New Anti-Fibrotic Drugs
Authors: Caroline S. Freathy, Samantha M. Maitland, Kathryn L. Bagot, Howard J. Loraine, Paul R. Murdock, Amanda J Woodrooffe
For more information about the show click here.