Human HEPATOPAC Kit

Type:Cell Products
SUBTYPE:HEPATOPAC Kits
SPECIES:Normal
APPLICATION & SPECIALITY USES:ADME-Toxicology Products and Services
Keywords:Cytotoxicity AssessmentHepatocytesMechanistic ToxMetabolic StabilityMetabolismMetabolite IdentificationPredictive Tox

Human HEPATOPAC® co-cultures are derived from highly characterized donors. Plates may be customized to be a single donor, pooled donor or multiple donors in different wells on the same plate.

Human HEPATOPAC kits are shipped with all necessary media and an instructions for use manual. Dosing can begin two days after receiving the kit.

Validation

Human HEPATOPAC co-cultures remain viable and highly functional for four weeks. The co-cultures secrete albumin, synthesize urea, display functional bile  canaliculi, and metabolize compounds using active Phase I and Phase II drug metabolism  enzymes. The following data illustrates the morphology, liver function, and metabolizing enzyme activity expected from human HEPATOPAC co-cultures.

Morphology

Human HEPATOPAC co-cultures were created and maintained in vitro for over four weeks. Over this time there were no substantial  changes to the morphology of micropatterned co-cultures and the polygonal shape was maintained. Transport of a fluorescent dye via MRP-2 demonstrates the robust network of bile canaliculi  between hepatocytes.

Morphology
Liver Function

The following chart compares albumin secretion in the HEPATOPAC co-culture versus hepatocytes in suspension, illustrating the long-term viability of the HEPATOPAC co-culture.

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Metabolizing Enzyme Activity

Various CYP and Phase 2 enzyme activities were in human HEPATOPAC co-cultures. Metabolites were assessed using mass spectrometry. The following charts illustrate that  HEPATOPAC co-cultures maintain metabolic activity over time.

co-culture-Applications-fig4-lg-2014-1