Immunoglobulin Inhibiting Reagent (Powder)

Type:Media / Reagents / Kits
SUBTYPE:Immunology Tools
APPLICATION & SPECIALITY USES:Biomarker DiscoveryPersonalized Medicine

OPTIONS:

A unique reagent to prevent heterophilic antibody interference in immunoassays, Immunoglobulin Inhibiting Reagent (IIR) is a formulation of immunoglobulins that is specifically targeted against heterophilic antibodies to block their interference in immunoassays. IIR is not precipitated IgG. Because of it’s unique formulation and it’s purity (>96%), IIR may be used at extremely low concentrations without any effect on dilutions or recovery.

IIR has an extremely high binding affinity which enables it to inhibit the action of human heterophilic antibodies. The affinity value of human heterophilic antibodies is in the order of 106 Litres/Mol. Immunoglobulin Inhibiting Reagent (IIR) has a binding affinity which exceeds 109 Litres/Mol. This greatly enhanced binding affinity ensures that heterophilic interference is virtually eliminated.

To read more about use of this reagent, click here.

Features & Benefits

  • Available in standard and custom sizes
  • Widely cited
  1. Todd DJ, Knowlton N, Amato M, et al. Erroneous augmentation of multiplex assay measurements in patients with rheumatoid arthritis due to heterophilic binding by serum rheumatoid factor. Arthritis Rheum. 2011;63(4):894-903.
  2. Zhang H, Fu T, Mcgettigan S, et al. IL-8 and cathepsin B as melanoma serum biomarkers. Int J Mol Sci. 2011;12(3):1505-18.
  3. Lapan P, Brady J, Grierson C, et al. Optimization of total protein and activity assays for the detection of MMP-12 in induced human sputum. BMC Pulm Med. 2010;10:40.
  4. Erickson JA, Lu J, Smith JJ, Bornhorst JA, Grenache DG, Ashwood ER. Immunoassay for quantifying squamous cell carcinoma antigen in serum. Clin Chem. 2010;56(9):1496-9.
  5. Kellar KL, Kalwar RR, Dubois KA, Crouse D, Chafin WD, Kane BE. Multiplexed fluorescent bead-based immunoassays for quantitation of human cytokines in serum and culture supernatants. Cytometry. 2001;45(1):27-36.
  6. Kellar KL, Kalwar RR, Dubois KA, Crouse D, Chafin WD, Kane BE. Multiplexed fluorescent bead-based immunoassays for quantitation of human cytokines in serum and culture supernatants. Cytometry. 2001;45(1):27-36.
  7. Nicholson S, Bomphray CC, Thomas H, et al. A phase I trial of idiotypic vaccination with HMFG1 in ovarian cancer. Cancer Immunol Immunother. 2004;53(9):809-16.
  8. Perlee LT, Christiansen J, Dondero R, et al. Development and standardization of multiplexed antibody microarrays for use in quantitative proteomics. Proteome Sci. 2004;2:9.
  9. Reinsberg J. Interferences with two-site immunoassays by human anti-mouse antibodies formed by patients treated with monoclonal antibodies: comparison of different blocking reagents. Clin Chem. 1998;44(8 Pt 1):1742-4.

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