Immunohistochemistry (IHC)

SUBTYPE:Protein Expression
APPLICATION & SPECIALITY USES:Biomarker DiscoveryCell Therapy/ Regenerative MedicineImmuno-oncologyPersonalized Medicine
Keywords:Antibody ValidationIHCImmunohistochemistryProtein Expression


Our PHASEZERO® scientists have over 20 years of experience performing human tissue-based IHC, with a specialty in custom assay development for commercially-available or client-generated antibodies and binding molecules (biosimilars, bi-specifics and antibody-drug conjugates). Our scientists apply rigorous approaches to deliver intelligent assay design, optimized and robust immunostaining protocols and expert data interpretation.

Features & Benefits

IHC assays are fully automated and run on the following machines:
• Dako Autostainer Plus (IQ/OQ/PQ validated)
• Ventana Discovery XT
• Leica BondRX machines

Facility and Capabilities
• GLP accredited for moderate throughput IHC
• Fully trained, experienced scientists included 3 GLP TCR Study Directors
• Consulatant Histopathologists – Clinical and Veterinary
• SOPs for complete process – Tissue Validation, Preparation, Assay, Analysis and Reporting
• Digital Pathology – Aperio ScanScope AT Turbo Image analysis, archiving and on-line peer review

• Wide experience of multiple detection systems and protocols
• Range of antigen retrieval approaches including high pressure/ high temperature
• Dako Envision, Envision Flex+, and CSAII™; Vector ABC and ImmPress™; and Menarini polymer HRP detection and fluorescent labeled secondary antibodies
• Alkaline phosphatase, horseradish peroxidase and glucose oxidase chromogenic endpoints
• Ventana Omnimap™ and Ultramap™ detection systems
• Leica bond polymer refine detection
• Multiple antibody species detection available including mouse, rabbit, goat, rat and chicken
• Multiplexing with chromogenic or fluorescent endpoints

• Fluorescence analysis and multiplexing (up to 7 targets simultaneously)
• Confocal and fluorescence microscopy
• Scalable via use of TMAs
• Semi-quantitative analysis
• Consultant Histopathologist support
• Image analysis
• Vectra Polaris image scans allows digital analysis and integrates seven-color multispectral imaging with whole-slide scanning
• Aperio Digital image scans allow digital analysis of full face and TMA slide sections
• ImageQuant – segmentation and TMA algorithms