in situ Hybridization

SUBTYPE:Gene Expression
APPLICATION & SPECIALITY USES:Biomarker DiscoveryCell Therapy/ Regenerative MedicineImmuno-oncologyPersonalized Medicine
Keywords:FISHGene Expression LocalizationISHVisualization of Gene Expression


Our PHASEZERO® division offers the latest generation branched-chain ISH technologies (ViewRNA® and RNAscope®), which represent a significant advance in sensitivity and throughput compared with traditional riboprobe ISH methods, for the detection and precise localization of cellular mRNA in human tissue. Ideally suited for target and biomarker validation and diagnostic assay development and testing, branched-chain ISH offers significant benefits.

Features & Benefits

  • Rapid and cost-effective, branched-chain ISH accelerates target and biomarker validation
    • Commercial availability of branched-chain ISH probes eliminates the need for additional up-front molecular biology work, required for traditional ISH.
  • Increased sensitivity reduces the risk of missing relevant expression patterns by enabling the detection of low-level mRNA production
    • Detecting extremely low levels of mRNA as compared with traditional ISH methodology, branched-chain ISH reduces the risk of false-negatives.
  • Highly-specific assays make use of FFPE tissue possible
    • Relying on the hybridization of large numbers of short oligonucleotide probe pairs, branched-chain ISH detects the less-well preserved mRNA in formalin-fixed, paraffin-embedded (FFPE) tissue as well as FFPE tissue microarrays (TMAs). Frozen sections can also be used and can offer greater sensitivity due to more complete RNA preservation.
  • ISH Multiplexing (ISH:ISH & ISH:IHC)
    • Via ISH:ISH multiplexing, PhaseZERO is able to localize the expression of multiple mRNA targets in the same tissue.
    • Via ISH:IHC multiplexing, PhaseZERO is able to localize mRNA and protein expression in the same tissue.
  • ISH Optimization, Validation, and Sensitivity
    • PHASEZERO’s in situ hybridization protocols allow for the precise localization of mRNA in human tissue. Utilizing customer-specific assay development, optimization and validation with either commercially available probes or custom target probes, our ISH offers additional levels of security in the data interpretation, validation of expression to known gene expression and high sensitivity detection capabilities.