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We identify / profile metabolites by incubating the test compound in triplicate in four species (human, mouse, rat, and dog) of suspended hepatocytes. We typically include the time points: 0, 15, 30, 60, 120, and 240 minutes. A positive and negative control is included. Samples are collected and stored at ≤ 70°C for analysis by clients who prefer to do analyses in-house by our partners using UPLC-MS/MS. Our report includes an analysis of sub-structural localization of the biotransformations observed.
Additionally, positive control samples are analyzed for disappearance of parent, to demonstrate the metabolic competence of the system.