SUBTYPE:Protein Expression
APPLICATION & SPECIALITY USES:Biomarker DiscoveryCell Therapy/ Regenerative MedicinePersonalized Medicine
Keywords:IHCImmune Cell-target VisualizationImmuno-OncologyImmunohistochemsitryImmunooncologyIO DevelopmentMultiplexMultiplex ImmunohistochemsitryVectra Polaris

With an extensive tissue bank, we specifically customize your project to your needs. Our PHASEZERO® team has working protocols for targeting a wide range of oncology targets, plus routine immune cell markers. We recently adopted the PerkinElmer Vectra® Polaris™ Automated Quantitative Pathology Imaging System to extend their services capabilities to drug and diagnostic development scientists working in the Immuno-Oncology (IO) field. Capitalizing on over 20 years of expertise in human tissue-based research, PHASEZERO scientists are excited to extend this additional solution platform to our clients.

The Vectra Polaris system integrates seven-color multispectral imaging with whole-slide scanning in a simplified digital pathology workflow to support the quantification and analysis of tissue sections that are stained with multiple immunohistochemical stains.

Development and validation of immuno-oncology (IO) targets requires an understanding of the biology occurring in the immune system and tumor. Multiplexed IHC can be effectively used to visualize cancer targets and multiple immune cell types within the tumor and its microenvironment (TME). Visualization of the spatial relationship and functional state of immune cell types with tumor cells is achieved through multiplex labeling of these different cell types and defining the location within the TME.

Cancer Targets Immune Markers

Phosphorylated targets

T cells NK Cells
Acetyl, phopho & total c-Myc IL-1R alpha  IL-33 CD3 pan T cells CD56
Phospho & total p44/42 MAPK (Erk1/2) CD123 KLF2 CD4 Th cells
Phospho & total c-MET  CD200 LRP6 CD8 Tc cells Neutrophils
Phospho & Total Her2 / Her3  CD276 MICA/B FpxP3 T reg cells CD15
Phospho-p38 MAP kinase 5T4 PD-1
Phospho-IRF CREB-2 TRPM8 B Cells Macrophages
Phospho-TrkA  GD3  VIPR1 CD19 CD68
Casein kinase 2  GITR  VIPR2 CD20cy
Heregulin 1 alpha  ICAM1 Ki67
HIF1 alpha  IDO-1 Mast cell tryptase

Features & Benefits

IHC assays are fully automated and run on the following machines:
• Dako Autostainer Plus (IQ/OQ/PQ validated)
• Ventana Discovery XT
• Leica BondRX machines

Facility and Capabilities
• GLP accredited for moderate throughput IHC
• Fully trained, experienced scientists included 3 GLP TCR Study Directors
• Consulatant Histopathologists – Clinical and Veterinary
• SOPs for complete process – Tissue validation, Preparation, Assay, Analysis and Reporting
• Digital Pathology – Aperio ScanScope AT Turbo Image analysis, archiving and on-line peer review

• Wide experience of multiple detection systems and protocols
• Range of antigen retrieval approaches including high pressure/ high temperature
• Dako Envision Flex+ , Dako Envision, Vector ABC, Vector ImmPress™ and Dako CSAII™ and Menarini polymer HRP detection and fluorescent labelled secondary antibodies
• Alkaline phosphatase, horseradish peroxidase and glucose oxidase chromogenic endpoints
• Ventana Omnimap™ and Ultramap™ detection systems
• Leica bond polymer refine detection
• Multiple antibody species detection available including mouse, rabbit, goat, rat and chicken
• Multiplexing with chromogenic or fluorescent endpoints

• Fluorescence analysis and multiplexing (2-3 targets simultaneously)
• Confocal and fluorescence microscopy
• Scalable via use of TMAs
• Semi-quantitative analysis
• Consultant Histopathologist support
• Image analysis
• Vectra Polaris image scans allows digital analysis integrates seven-color multispectral imaging with shole-slide scanning
• Aperio Digital image scans allow digital analysis of full face and TMA slide sections
• ImageQuant – segmentation and TMA algorithms