Rat HEPATOPAC Kit

Type:Cell Products
SUBTYPE:HEPATOPAC Kits
SPECIES:Rat
APPLICATION & SPECIALITY USES:ADME-Toxicology Products and Services
Keywords:Cytotoxicity AssessmentHepatocytesMechanistic ToxMetabolic StabilityMetabolismMetabolite IdentificationPredictive Tox

Rat HEPATOPAC® co-cultures are micropatterned on industry-standard plates which can be customized for various applications and to meet size requirements.

Rat HEPATOPAC kits are shipped with all necessary media and an instructions for use manual. Dosing can begin two days after receiving the kit.

Validation

Rat HEPATOPAC co-cultures remain viable and highly functional for four weeks. The co-cultures secrete albumin, synthesize urea, display functional bile  canaliculi, and metabolize compounds using active Phase I and Phase II drug metabolism  enzymes. The following data illustrates the morphology, liver function, and metabolizing enzyme activity expected from rat HEPATOPAC co-cultures.

Morphology

Rat HEPATOPAC co-cultures were created and maintained in vitro for over four weeks. Over this time there were no substantial  changes to the morphology of micropatterned co-cultures and the polygonal shape was maintained. The following pictures illustrate the morphology at days 7 and 20 of a rat HEPATOPAC co-culture and the formation of bile canaliculi at days 7 and 13.

Rat_Morphology
Rat_Bile

 

Liver Function

The following charts show albumin secretion and urea synthesis in a rat HEPATOPAC co-culture over time, illustrating the long-term viability of the HEPATOPAC co-culture.

Rat_Albumin_and_Urea

Metabolizing Enzyme Activity

Various CYP and Phase 2 enzyme activities were measured in rat HEPATOPAC co-cultures. Metabolites were assessed using mass spectrometry. The following charts illustrate that HEPATOPAC co-cultures maintain metabolic activity over time.

Rat_CYP_profile