Using our qRT-PCR platform we provide robust, quantitative gene expression data in standard and custom cohorts of diseased and non-diseased tissues. The data is used to support target selection, to understand tissue specific, disease relevant or population differences in gene expression. Some additional purposes include to alert investigators to potential safety liabilities and to validate animal models. We are also able to characterize tissue cohorts for specific somatic mutations, like KRAS, EGFR, AKT using a qRT-PCR based approach.
PHASEZERO offers custom PCR analysis for clients who wish to investigate the level of expression of a target(s) in cohorts of specific normal and/or diseased tissue types. Tissues used can be specific by the client (prospective collection, BioIVT sourced or client-owned). Our scientists can start with the tissue specimens and preform the DNA/RNA extraction or start with client supplied DNA/RNA. We then QC the nucleic acid and perform the qRT-PCR.
Features & Benefits
- Design of TaqMan® and MGB primer probes
- Low density arrays
- RNA extraction and quality control
- XPRESSWAY™ profiles or custom gene expression profiling