FAQ - STORAGE
When stored at the proper storage conditions (< -150° C), the cytochrome P450 enzyme systems remain unchanged after more than 5 years of storage.
No, the cryoshipper is not intended for storing cryopreserved hepatocytes. Upon receiving your shipment the cryopreserved hepatocyte vials must be removed and stored at temperatures less than -150°C.
The cells should be stored in liquid nitrogen – vapor phase < -150° C.
Media: (2° to 8° C) Do not freeze media. Storing media refrigerated maximizes shelf life.
Antibiotics: (-15° to -25° C) Do not store in refrigerator. Avoid storage in "Frost-Free" freezer as temperature cycles can damage product.
Microsomes/S9: (-70° to -90° C) Store in mechanical freezer. Limit number of freeze/thaw cycles.
Hepatocytes: (< -150° C) Store in vapor phase of liquid nitrogen freezer. Do not allow product to thaw until ready for use. Do not store in liquid phase of liquid nitrogen freezer or Dewar type flask.
These products should always be stored in an ultracold freezer at temperatures < -70° C. Stored under these conditions, the cytochrome P450 enzyme systems are remarkably stable, showing little, if any, loss of activity after storage for more than 5 years. However, some of the phase II enzyme systems, particularly those in S9 such as N-acetyltransferase, are not stable even at -70° C, and lose significant activity within a matter of weeks or months.