Posters

BioIVT presented posters at various meetings. Below is a list of recent poster presentations.

Predicting Drug-induced Liver Injury In Vitro
Presented at the 39th Annual Meeting of the American College of Toxicology (ACT), November 4 – 7, 2018, this poster describes a study in which BioIVT collaborated with Biogen to assess the hepatotoxicity potential of four preclinical drug candidates using the C-DILI™ Assay. Study results were consistent with findings from rodent studies and predicted that one of the compounds has the potential to cause cholestatic hepatotoxicity in humans.


Characterization of Hepatobiliary Transport of a Bile Acid In Vitro to Identify its Rate-Determining Process; Use of Opti-Expression Technology
Presented at the 33rd JSSX Annual Meeting, October 1 – 5, 2018, this poster demonstrates the utility of OPTI-EXPRESSION™ Technology to mimic in vivo expression of hepatic transporters.


Predicting Hepatotoxicity Integration of Transport Metabolism and Regulation of Bile Acids 
Presented at the EuroTox 2018 Conference, this poster presents new research that describes the mechanisms associated with cholestatic drug-induced liver injury.


An Integrated In Vitro Screen Using Sandwich-Cultured Human Hepatocytes for Prediction of Cholestatic Hepatotoxicity
Presented at the 2018 North American ISSX Meeting.


Development of a Novel Human 3D In Vitro Model for Evaluating New Anti-Fibrotic Drugs
Presented at the 2018 Advances in Cell and Tissue Culture event this poster explores the development of the liver ORGANDOT™ model for the efficacy testing of new anti-fibrotic drug candidates.


C-DILI™ Assay: Integrating BSEP Inhibition and FXR Antagonism to Improve Prediction of Cholestatic Drug-Induced Liver Injury
Presented at the AAPS Joint Workshop on Drug Transporters in ADME: From The Bench To The Bedside.


An Image-Based Method to Detect and Quantify T Cell-Mediated Cytotoxicity of 2D and 3D Target Cell Models
Presented at AACR 2018 in collaboration with BioTek.


Prediction of Clinical Hepatotoxicity of Clinical Drug Candidates using the Novel C-DILI Assay_A Retrospective Case Study
Presented at the 2018 SOT meeting.


Hepatobiliary Disposition of 15 Non-Therapeutic Chemicals in Sandwich-Culture Rat Hepatocytes using B-CLEAR® Technology
Presented at the 2018 SOT meeting.


Multi-parameter Microtiter Assay to Screen Anti-HBV Agents Using Primary Human Hepatocytes
Presented at the 2017 International HBV Meeting held in Washington, DC.


Utilizing Multiplex Chromogenic IHC and Digital Image Analysis to Evaluate Immune Cell Content and Spatial Distribution within NSCLC Tumor Tissue
Presented at the 2017 AACR


Content and Spatial Distribution Analysis of PD-L1 Positive Tumor Cells and CD8 Positive T-cells Within Multiplex IHC Stained NSCLC Tumor Tissue
Presented at the 2017 Molecular Medicine TriConference


Development of Robust in vitro 3D Models of Human Tumors for Identification and Evaluation of Anti-cancer Drugs
Presented at the AACR Int. Conf on New Frontiers in Cancer Research, 2017


Development of a Novel Micropatterned Co-Culture Model with Primary Dog Hepatocytes to Aid in Accurate Prediction of DILI Risk 
Presented at the 2017 SOT Conference this poster presents results that validate the dog HEPATOPAC model for assessing clinical DILI.


Rapid and Low-Cost Assessment of Compound DDI Potentials using MDCK-II Cells Co-Expressing Regulatory Transporters OATP1B1, OATP1B3, OAT1, OAT3, OCT2, P-gp, BCRP and MATE1
Presented at the 3rd ITC Workshop 2017. This poster describes the use of multi-transporter models (created with OPTI-EXPRESSION Technology) for accurate and lowcost substrate assessment for eight common DDI transporters.


Development of a Novel Histochemical Binding Assays - Unique Challenges for Unique Molecules
Presented at the 11th European Antibody Congress


Development of a Novel Approach for Immunohistochemical Assay Validation
Presented at the 2015 Molecular Medicine TriConference


Identification of Major Primary and Secondary Metabolites of Selected Drugs in Dog Micropatterned Hepatocyte Co-Cultures Using LC-MS/MS Acquisition: Correlation with In Vivo Human Metabolism
Presented at the 2016 ISSX Conference in Boston this poster presents data that highlights the superiority of a long-term, functional tissue engineered liver model such as the HEPATOCPAC platform, over traditional models in correlating in vitro and in vivo species specific metabolites and in identifying and predicting clinically-relevant metabolites.


In Vitro Evidence of OATP1B1-Induced Drug-Serum Protein Binding Shift
and Its Implications on Predicting Drug Clearance and Drug-Drug Interactions

Presented at the 2016 ISSX Workshop. The poster describes data that substantiate previous experimental findings and TIPBS hypothesis (see poster presented in 2015). The data demonstrate that both OATP1B1-mediated transport of highly protein-bound substrates in human serum, and their inhibition by Rifampicin, can be underestimated using conventional approaches.


Development and Validation of the Human isletOrganDOT™ 3D Culture System for Evaluation of Insulin Secretagogues
Presented at the 2015 Advances in Cell & Tissue Culture Conference


In Vitro Modeling of Inflammation-Drug Interactions Using Micropatterned Co-Cultures of Primary Hepatocytes and Kupffer Macrophages
Presented at the 2015 SOT Conference this poster describes how HepatoMune co-cultures may be used to predict drug induced liver injury mediated by inflammatory stress.


Nuclear Receptor-Mediated Changes in Gene Expression in a Human Hepatic Micropatterned Co-Culture Model Following Treatment with Hepatotoxic Compounds
Presented at the 2015 SOT Conference in collaboration with The Hamner Institute, this poster describes a study evaluated activity of nuclear receptors in human HEPATOPAC model.


Multiplexed Method to Screen for Chemical-Induced Hepatotoxicity Using a Novel Micropatterned Human Hepatocyte Co-Culture Platform
Presented at the 2015 SOT Conference in collaboration with The Hamner Institute, this poster describes a study to determinet the fidelity of multiplexed high-content screening endpoint assays in human HEPATOPAC co-cultures for MOA-based screening.


A Novel In Vitro Model to Assess Chemical-Induced Steatosis and Phospholipidosis
Presented at the 2015 SOT Conference in collaboration with The Hamner Institute, this poster describes a study to utilize HEPATOPAC co-cultures to develop a novel high-content screening strategy that can identify a compound’s potential for alteration of human lipid dynamics and metabolism.


In Vitro Toxicity Assessment of the Nucleoside Analog Fialuridine Using Micropatterned Primary Hepatocyte Cocultures And Discovery of a Non-Toxic Isomer
Presented at the 2015 SOT Conference in collaboration with Janssen Research and Development this poster describes a study which showed the utility of HEPATOPAC co-cultures to predict the toxicity of fialuridine, a drug that failed in clinical trials due to hepatotoxicity.


Phenotypic Assessment of Toxicity Using a Human Hepatocyte Co-Culture Model
Presented at the 2015 HCA Conference in collaboration with Molecular Devices, LLC, this poster describes a method to use high-content imaging assasys with HEPATOPAC models.


Utility of Pooled Cryoplateable LIVERPOOL in the In Vitro Determination of Cytochrome P450 Metabolism and Induction
Presented at the 20th North American ISSX Meeting


Transporter-Induced Protein Binding Shift (TIPBS) Hypothesis and Modeling
Presented at the 20th North American Regional ISSX Meeting in 2015. This poster hypothesizes (and attempts to quantitatively explain) how transporter-induced changes in protein binding can cause a drug's kinetic parameters to be underestimated, and contribute to discrepancies in predicting transporter-mediated drug clearance and DDIs.


Anti-HIV Protease Inhibitors May Aggravate Rifampicin Induced Liver Injury Through Multifaceted Interactions on Hepatic Transporters
Presented at the 116th ASCPT Meeting in 2015. This poster shows that liver toxicity induced by rifampicin is likely aggravated by co-medication with protease inhibitors, which further inhibit bile salt transporters, resulting in increased levels of rifampicin in the liver.


In vitro-In vivo Correlation (IVIVC) of Drug Induced Inhibition of Creatinine Tubular Secretion using MDCK Cells Expressing OCT2/OAT2/OCT3OCT3/MATE1/MATE2K Transporters
Presented at the 116th ASCPT Meeting in 2015. This poster presents our novel proximal tubule creatinine secretion model, in which polarized MDCK-II cells co-expressing five key transporters are used to assess the effects of seven drugs on creatinine secretion; data show a close correlation between in vitro and in vivo data.


Validation of a 3-Dimensional Liver Microtissue Model for Long Term Hepatotoxicity Studies
Presented at the 2014 Society of Toxicology Annual Meeting in Phoenix, Arizona


Evaluation of the Toxicity Profiles of Selected Bioactivated Compounds in Primary Rat Hepatocytes Cultured in Micropatterned Co-Cultures
Presented at the 2014 SOT Conference this poster provides data from an assessment of the bioactivation and cytotoxicity of acetaminophen and other compounds using a 96-well rat HEPATOPAC model.


Toxicity of Selected Bioactivated Compounds in Primary Rat Hepatocytes Cultured in Micropatterned Co-Cultures
Presented at the 2013 EuroTox Conference in collaboration with The Hamner Institute, this poster describes a study that assessed bioactivation and cytotoxicity of acetaminophen in the 96-well HEPATOPAC format.


Comparative Metabolism of Eight Model Pharmaceutical Compounds in Rat and Human Liver Microsomes, Suspension Hepatocytes and Micropatterned Co-cultures of Primary Hepatocytes
Presented at the 2014 SOT Conference in collaboration with Ricerca, this poster demonstrates that HEPATOPAC co-cultures are more metabolically active than other in vitro hepatocyte models.


Measuring Intrinsic Kinetic Constants for P-glycoprotein-Mediated Quinidine Efflux Transport Using Intracellular Unbound Concentration in MDR1-MDCKII Cells
Presented at the 19th ISSX Meeting. The poster demonstrates the importance of determining intrinsic kinetic parameters over apparent kinetic parameters, and shows that simulations based on intrinsic kinetic parameters produced more reliable predictions than those based on apparent kinetic values.


Novel High-Throughput Method for Measuring Intracellular Unbound Fraction
Presented at the 19th North American ISSX Meeting. The poster presents a novel high-throughput method for measuring the intracellular fraction of unbound drugs.


False Negatives May Occur in BCRP Substrate Assessments Depending on the In Vitro Assay System Used
Presented at the 2014 AAPS Annual Meeting. The poster demonstrates that although different in vitro assay systems can produce false positives in BCRP substrate studies, our triple transporter (OATP1B1/1B3/BCRP) model was the most effective in identifying BCRP substrates.


The Correlations between Transporter Protein Expression, Apparent Transcellular
Permeability and Intrinsic Efflux Permeability: A Case Study on BCRP/ABCG2 Transporter

Presented at the 19th North American ISSX Meting. This poster shows the linear relationship between the DNA concentrations used in our transient transfection system and (1) the resulting BCRP protein expression levels, and (2) the intrinsic efflux permeability; and reinforces the suggestion that the intrinsic efflux permeability be used, instead of the apparent permeability, to evaluate the efflux kinetics of ABC transporters.


A Micropatterned Culture with Primary Hepatocytes and Kupffer Macrophages for Studying Inflammation-Drug Interactions
Presented at the 2013 SOT Conference this poster presents data that illustrates how rat or human HEPATOPAC-Kupffer cell co-cultures may be used to predict drug induced liver injury mediated by inflammatory stress.


In Vitro Modeling of Cytokine- Drug Interactions Using Micropatterned Co-Cultures of Primary Hepatocytes and Kupffer Macrophages
Presented at the 2013 EuroTox Conference this poster illustrates an evaluation of cytokine-drug interactions in which HEPATOPAC co-cultures were supplemented with Kupffer cells.


A Micropatterned Culture with Human Hepatocytes And Kupffer Macrophages For Studying Inflammation-Drug Interactions
Presented at the 2013 AAPS Conference this poster shows how rat or human HEPATOPAC- Kupffer cell co-cultures may be used to predict drug induced liver injury mediated by inflammatory stress.


Bioactivation and Toxicity of Acetaminophen in Rat Primary Hepatocytes Cultured in Micropatterned Co-Cultures
Presented at the 2013 SOT Conference in collaboration with The Hamner Institute, this poster describes a study that assessed bioactivation and cytotoxicity of acetaminophen as a function of culture age in the 96-well HEPATOPAC format.


Comparison of Inhibition of CYP1A2, 2C9 and 3A4 using Human Liver Microsomes and Hepatocytes
Presented at the 10th International ISSX meeting


Use of the CYP3A4 Selective Inhibitor CYP3cide in CYP3A5 Genotyped Cryopreserved Human Hepatocytes to Explore the Individual Contribution of CYP3A4 and CYP3A5 in Drug Metabolism
Presented at the 10th International ISSX meeting


Demographic Differences by Age, Gender, BMI and Ethnicity of Phase I and II Enzyme Activities in Cryopreserved Human Hepatocytes
Presented at the 2013 Society of Toxicology Annual Meeting in San Antonio, Texas


Development and Characterization of a Quintuple Transporter Model for Studying Tubular Creatinine Secretion
Presented at the 2013 AAPS workshop. This poster details the development of our quintuple transporter, proximal tubule creatinine secretion model.


Transporter Inhibition by Herbal Supplements--Potential Drug-Dietary Supplement Interactions
Presented at the 2013 ISSX Meeting. The poster investigates potential herbal supplement-drug interactions; several common herbal supplements were tested on transporters known to be involved in drug-drug interactions.


Comprehensive Profiling of Inhibitory Effects on Major CNS Transporters for Drug Safety Assessment
Presented at the 2013 ACT Meeting. The poster details the screening of 30 CNS drugs against EAAT2--a major transporter responsible for glutamate recyling. Also detailed here is the screening of a small llibrary of amino acid analog neurotoxins against transporters related to glutamate signaling, including EAAT1-3, xCT, asc-1 ,and ASCT2.


Comprehensive Assessment of Inhibitory Effects of CNS-Acting Drugs on Major Neurotransmitter Transporters
Presented at the 2013 AAPS Meeting. The poster details the development of an assay platform for the systematic assessment of the inhibitory effects of drugs on 15 key neurotransmitter transporters in order to better understand the therapeutic and adverse effects of drugs on the CNS.


Functional Assessment of OATP1B1 and BCRP Polymorphisms in an OATP1B1/BCRP Co-Expressing Model
Presented at the 2013 ISSX Meeting. The poster demonstrates novel OATP1B1/BCRP co-expressing cellular models that are useful tools for studying the vectorial transport of drugs and the effects of transporter
polymorphisms.


Studying Drug-Induced Cholestasis with a Novel Cellular Model Co-Expressing the Major Bile Salt Transporters in the Liver
Presented at the 2013 SOT Meeting. The poster details the use of an in vitro cholestasis assay model, in which three transporters--OATP1B1, NTCP and BSEP--are coexpressed in polarized MDCK cells, and demonstrates the utility of this novel model in evaluating drug-induced liver damage.


Use of Hepatocyte and Kupffer Cell Co-Culture Models in Assessment of Cytochrome P450 Metabolism
Presented at the 2012 North American ISSX Meeting in Dallas, Texas


Selective Conjugation of 7-hydroxycoumarin by Recombinant Human Uridine 5'-diphospho-glucuronosyltransferase (UGT)
Presented at the 2012 European ISSX MDO Meeting in Noordwijk, The Netherland


A Micropatterned Culture with Human Hepatocytes And Kupffer Macrophages For Studying Inflammation-Drug Interactions
Presented at the 2012 SOT Conference this poster describes augmenting the HEPATOPAC platform with primary Kupffer macrophages in order to mimic A component of inflammation.


A Micropatterned Culture with Human Hepatocytes And Kupffer Macrophages For Studying Inflammation-Drug Interactions
Presented at the 2012 ISSX Conference this poster describes development of an in vitro hepatic model to mimic inflamation.


Assessment of a Micropatterned Hepatocyte Co-Culture System to Detect Compounds that Cause Drug-Induced Liver Injury in Humans
Presented at the 2012 SOT Conference in collaboration with Pfizer, Inc., this poster describes use of HEPATOPAC co-cultures to predict DILI for 45 compounds for which hepatotoxicity is known.


Enhancement Of Proliferation in a Novel Rat Hepatocyte Co-Culture Model After Mitogenic Stimulation
Presented at the 2012 SOT Conference in collaboration with the US EPA, this poster describes a study in which rat HEPATOPAC co-cultures were compard to rat hepatocyte monocultures under various conditions.


Functional comparisons of human and rat concentrative nucleoside transporters CNT1, CNT2 and CNT3
Presented at the 2012 AAPS Meeting. This poster compares the functional activities of human and rat CNT1, CNT2 and CNT3 transporters, and profiles the inhibitory effects of major nucleoside and nucleotide analog drugs on these transporters in order to identify interspecies differences.


Functional Characterization of Major Solute Carrier (SLC) Transporters Involved in Drug-Drug Interactions Using a Polarized MDCK Model
Presented at the 2012 ISSX Meeting. The poster characterizes the over-expression and function of clinically-relevant SLC drug transporters transiently expressed in polarized MDCK cell models, and demonstrates the robustness of these models for use in DDI studies.


Changes in Phase I and II Enzyme Activities Over Time In Plated Human Hepatocytes
Presented at the 2011 SOT Annual Meeting & ToxExpo, Washington, D.C.


Micropatterned Primary Hepatocyte Co-Cultures for Drug Metabolism and Toxicity Studies
Presented at the 2011 SOT Conference this poster demonstrates the utility of HEPATOPAC co-cultures for long-term ADME Tox studies.


Micropatterned Primary Hepatocyte Co-Cultures for Drug Metabolism and Toxicity Studies
Presented at the 2011 SOT Conference this poster demonstrates the utility of HEPATOPAC co-cultures for long-term metabolism and toxicity studies.


Global Gene Expression Changes Induced In Primary Human Hepatocytes By Thiazolidinediones Upon Repeat Dosing of HEPATOPAC® Cultures
Presented at the 2011 ISSX Conference this poster illustrates how HEPATOPAC co-cultures may be uses to assess gene expression in hepatocytes.


A Long Term Culture Model for Primary Hepatocytes from Cynomolgus Monkeys
Presented at the 2011 ISSX Conference this poster describes development of the monkey HEPATOPAC co-culture model.


Assessing Chronic Toxicity of Fialuridine in A Micropatterned Hepatocyte Co-culture Model
Presented at the 2011 ISSX Conference this poster describes a collaboration with Alnylam Pharmaceuticals in which HEPATOPAC co-cultures were used to evaluate and predict fialuridine hepatotoxicity.


Accurate Prediction of Hepatic Clearance for Low-Clearance Compounds Using Micropatterned Hepatocyte-Stromal Cell Co-Cultures (HEPATOPAC®)
Presented at the 2011 ISSX Conference in collaboration with Boehringer Ingelheim Pharmaceuticals, Inc., this poster demonstrates the utility of HEPATOPAC for the prediction of in vivo hepatic clearance of low-clearance compounds.


Evidence for CYP2c9:CYP3a4 Enzyme Activity Interactions in Human Hepatocytes Through Modulation of Enzyme Levels
Presented at the 2011 ISSX Conference in collaboration with Boehringer Ingelheim Pharmaceuticals, Inc., this poster describes a study in which HEPATOPAC co-cultures were used to demonstrate that modulation of CYP3A4 expresssion can lead to appreciable changes in CYP2C9 activity.


Identification of Human Hepatotoxicants by High Content Imaging in Micropatterned Human Hepatocyte Cocultures
Presented at the 2011 SOT Conference in collaboration with Boehringer Ingelheim Pharmaceuticals, Inc., this poster describes a study in which HEPATOPAC co-cultures were used to study known human hepatotoxicants.


Be Careful What You Ask for: Challenges of Predicting Human Clearance for a Low Metabolic Turnover Compound, ELND006
Presented at the 2011 ISSX Conference this poster describes a collaboration with Elan Pharmaceuticals in which ELND006, a low clearance compound was evaluated in HEPATOPAC co-cultures as well as in other systems.


A Micropatterned Human Hepatocyte Co-Culture Model Allows Determination of Toxicity at More Relevant Concentrations than Hepatocyte Sandwich Cultures
Presented at the 2011 SOT Conference in collaboration with sanofi-aventis, this poster describes a stduy that demonstrated the extended viability of HEPATOPAC co-cultures enables determination of toxicity at more therapeutically-relevant doses that is possible in other models.


Implication of Genetic Polymorphisms in CYP2C9 and CYP2C19 on Drug Metabolism in Isolated Cryopreserved Human Hepatocytes
Presented at the 9th International ISSX Meeting, Istanbul, Turkey


Effects of Rosiglitazone and Sumatriptan on Human Isolated Small and Large Coronary Arteries
Presented at the 2008 EPHAR


Effects of Tagaserod on Human Isolated Coronary Arteries
Presented at the 2008 Safety Pharmacology Society Meeting


Effect of Alosetron and Tegaserod at the 5HT2B Receptor in Human Colon
Presented at the 2005 Digestive Diseases Week Meeting


Effects of JNJ-17333030, Naratriptan and Sumatriptan on Human Isolated Coronary Arteries
Presented at the 2007 Safety Pharmacology Society Meeting


Pharmacological Characterization of NECA-induced Fluid Secretion in Human Colon Mucosa
Presented at the 2004 British Pharmacological Society Meeting


G-CSF and GM-CSF are Differentially Released from Primary Human Lung Cells in Response to Pro-inflammatory Cytokines.
Presented at the British Society of Inflammation, 2004


Adenosine A2a Receptors are Responsible for NECA-induced Vasodilatation in Human Isolated Middle Cerebral Arteries
Presented at the 2003 British Pharmacological Society Meeting


PGN 1091, a Novel 5- HT2b Receptor Antagonist for the Treatment of Irritable Bowel Syndrome
Presented at the 2003 Digestive Diseases Week Meeting


Cytotoxicity of the Thiazolidinedione Compounds Troglitazone, Rosiglitazone and Pioglitazone in Isolated Human Hepatocytes
Presented at the 2000 British Pharmacological Society Meeting


Visualization and Characterization of Functional 5HT Receptors in the Human dorsal Raphe Nucleus by [35S]-GTPγS Autoradiography
Presented in the  European Journal of Neuroscience, 2000