BioIVT produces subcellular fractions through the process of subcellular fractionation which is the separation of cellular components. The initial step starts with the homogenization of tissue in an isotonic buffer to aid in the separation of the cellular components. Through differential centrifugation BioIVT is able to isolate purified organelle fractions for customer use like microsomes, S9, cytosol, lysosomes and mitochondria.
Microsomes and S9 are utilized in metabolism assays to study phase I and II enzymes. BioIVT manufactures a wide variety of options when it comes to subcellular fractions. Our standard product offering includes microsomes and S9 from human and animal liver tissue. However, custom preparations using other tissue types like brain, kidney, lung, intestine, heart and skin are requested often.
Our subcellular fractions are produced from fresh whole tissues to ensure the highest level of enzyme activity. The following subcellular fractions can be prepared for customers.
These subcellular fractions can be customized to your specifications:
Modified buffer formulations
Protein concentration (mg/mL)
Customer supplied tissue
Matched sets (microsomes, S9 and cytosol)
Subcellular fractions can be derived from commercially available animals.